PCR技术在水体微生物检测中的应用

聚合酶链式反应(PCR)技术因其特异性强、灵敏度高以及快速简便等优点广泛应用于水体微生物的检测。综述了PCR技术的原理、方法及其在水体微生物检测中的研究进展。主要介绍了该技术应用于水环境中微生物病原体的检测、污水生物处理过程的监测与评价中的重要意义以及本实验室在该领域的研究进展。PCR技术为在分子水平上进行环境微生物的结构、多样性分析提供了有力的实验手段,有着广泛的应用前景。     

地下水中病原菌及其快速检测技术研究进展

全世界尤其在发展中国家由地下水病原菌导致的环境安全问题日益频发,成为影响人群健康的重要因素。传统的病原菌检测技术存在费时、灵敏度低以及难以准确定量等问题,无法满足对地下水中种类日益增多的病原菌的有效监测。近年来,地下水中病原菌检测技术不断发展,其中聚合酶链反应技术、生物芯片及生物传感器等检测技术成为研究热点,在这些技术的基础上,以能实现微型便携、快速灵敏、准确特异和经济方便为目的的新材料和不同技术手段的整合技术成为地下水中病原菌检测发展的新方向。文章对近年来病原菌快速检测新技术进行综述,以期为受污染的地下水病原菌的有效监测和控制提供技术支持。     

六安流动地磁测量异常的映震能力探讨

将安徽省六安地区流动地磁测线 12个流动地磁测点的∑ |δfi|异常与安徽中西部地震活动性作对比分析 ,初步结果为 :12个流动地磁测点的∑ |δfi|值较大 (∑ |δfi|≥Fu) ,一年内安徽中西部地区的地震活动性增强 ;反之 ,则安徽中西部地区的地震活动性较弱。此方法在地震的中短期预报中有一定的实用意义。     

新时期完善全链条环评管理制度的建议

环境影响评价（以下简称“环评”）制度作为生态环境源头预防的重要手段，有力支撑生态文明建设、推进生态环境治理能力现代化。当前，我国已建立了包括生态环境分区管控，项目环评、规划环评，以及区域发展战略（政策）评估的环评体系和排污许可管理制度体系，但是全链条环评管理在制度联动、衔接融合和管理闭环等方面仍存在薄弱环节。为进一步健全生态环境源头预防制度体系，须加快构建功能定位明确、责任边界清晰、衔接关系顺畅的全链条环评体系；建立生态环境分区管控与环评管理、排污许可等制度之间“四联动、三融合、两闭环”的基本模式。建议进一步完善全链条管理制度顶层设计，推动重点领域科技攻关，深化政策创新试点，强化能力建设，为推进美丽中国建设提供支撑保障。     

An improved grey wolf optimizer algorithm for identification and location of gas emission

Identification of the leakage of hazardous gases plays an important role in the environment protection, human health and safety of industry production. However, lots of current optimization algorithms, such as particle swarm optimization (PSO) and Grey Wolf Optimizer (GWO), suffer from poor global optimization capability and estimation accuracy. In this work, a hybrid differential evolutionary and GWO (DE-GWO) algorithm is proposed. Tested by simulation cases and Prairie Grass emission experimental data, DE-GWO shows higher estimation accuracy than GWO. Compared with the other four optimization algorithms, DE-GWO exhibits finer robust stability under different population sizes, fewer iterations, as well as higher estimation accuracy with fewer search agents. Importantly, simulation results demonstrate that DE-GWO is more suitable to apply in the scene with a small number of sensors. Therefore, the proposed in this paper outperforms other optimization algorithms for the gas emission inverse problem. DE-GWO can provide reliable estimation towards gas emission identification and positioning, which shows huge potential as the data analysis module of real-time monitoring and early warning system.     

Based on material flow analysis: Value chain analysis of China iron resources

Iron is an important basic resource for national economic development in China. It is of great strategic importance for the sustainable development of China's economy to study the utilization and circulation status of iron resources. In this paper, using the material flow and value chain analysis method, we quantitatively analyzed the value flow of iron resources in China. According to the value chain and price theory of element M, a value stream diagram of iron resources corresponding to the substance flow chart was plotted. Based on the previous material flow analysis result of iron resources, the diagram quantitatively depicted the value of the circulating flow of iron resources in China in 2011. The results show that by recycling materials, the value of the circulating flow of iron resources can bring considerable economic benefits to both producers and consumers. In the production stage, the expenditures of the entire economic system was reduced by 91.77 billion RMB by circulating iron and the income increased by 95 billion RMB by recycling home scrap, which was generated in the crude steel production stage. In the use stage of iron and steel products, the recycling of old scrap enabled the entire economic system to recover 370.78 billion RMB. It should be noted that analysis within a single framework of physical and economic characteristics of iron resources in the economic system can further extend the research chain of substance flow and value flow at the macro level, enhancing the economic value of substances flow research. In addition, by tracking and depicting the value flow cycle of elements, the improvement potentials and the value situations can be determined to provide useful information for conducting processing and technological innovation for waste minimization.     

C、D、M_f值综合异常交汇区在大华北地区中强震前的短期异常变化

华东和华北区 1 970～ 1 998年 2 6个MS≥ 5.0地震前 2 3个地震存在C、D、Mf 综合异常区的中期异常。本文在此基础上 ,研究了这些异常在一年内的中短期变化。认为Mf 异常面积的短期变化最为显著 ,2 3个存在中期异常的地震中 2 1个地震前 6个月有 |η|≥ 0 .4的短期变化 ,其中 63%震前出现过地震活动平静 ,还有 37%为持续增强。最后用岩石刚度和压机刚度之比对岩石破裂前声发射的平静期长短的实验结果作了初步解释。     

台站短水准测量图件辅助管理系统简介

利用Access97编制了《台站短水准测量图件辅助管理系统》程序。本文简要介绍了该程序的设计思路、主要功能及运用效果     

Socially optimal and fund-balanced advanced recycling fees and subsidies in a competitive forward and reverse supply chain

Advanced recycling fees (ARFs) and government subsidy fees are important for curtailing the consumption of new products and encouraging recycling and disposal of end-of-life (EOL) products. We introduce a model consisting of a leader (the Environmental Protection Agency, EPA) and two groups of followers (MIS firms and recyclers) consisting of manufacturers, importers and sellers, and recyclers which compete in both consuming and recycling markets. The EPA determines the ARFs paid by the MIS firms and the fees subsidizing recyclers to maximize the social welfare in closed-loop supply chains where the MIS firms and recyclers attempt to maximize their respective profit functions. To compare with current practice, we describe a conceptual fund balance model to determine the ARF and subsidy fee on the basis of the balance between total collected ARFs and expenditure of subsidies. Using numerical examples for the laptop computer market in Taiwan, we demonstrate that our results outperform the current practice.     

城市PM2.5扩散网络模型的研究

以复杂网络理论为基础,运用最短增广链算法构建城市PM2.5扩散的容量网络模型。通过分析城市环境中水平距离、海拔、风力等因素的作用,对PM2.5区域性扩散的物理过程进行一次有益的探索。西安市近期空气监测数据的实证分析显示,预测值与监测值基本吻合。同时,模型输出揭示了西安市PM2.5的扩散途径。